Learn how REDIchips increase the dynamic range of your MALDI platform:
MALDI-MS has significantly transformed large molecule analysis, but due to matrix cluster peaks, analysis of low molecular weight compounds (below 500 Da) is limited. Successful screening and quantitation of small molecules with MALDI-MS requires careful choice of an appropriate matrix for a given molecule to minimize interfering matrix clusters. These issues are eliminated using a matrix-free approach, where silicon nanopost arrays are used for sample desorption, vaporization, and ionization (Figure 1). The nanopillar dimensions have been optimized for enhanced ion production of low molecular weight compounds by effectively coupling ultraviolet laser light via a resonance-like behavior, allowing for a matrix-free approach for small molecule analysis.
Matrix-free Small Molecule Analyses Using REDIchip on a MALDI Platform
Standard MALDI techniques are not ideal for small molecule analysis and detection in the lower spectral mass region (m/z < 1000). MS signals for small molecules are often masked or suppressed due to matrix interference.
REDIchips (Resonance-Enhanced Desorption Ionization) are sample target plates with nanopost array (NAPA) surfaces for matrix-free laser desorption ionization of small molecules. REDIchips eliminate the need for matrix application on your samples, enabling effective detection of small molecules and mixtures on your MALDI platform.
Metabolomic Profiling of Biofluids Using Laser Desorption Ionization on Nanopost Array Devices (REDIchips)
In this work, biofluid samples (urine) are processed using a solid phase extraction (SPE) method that enriches for polar and non polar small molecules which include glucuronides. Classification of gender from glucuronide analysis excreted in human urine has been reported in the past using nuclear magnetic resonance (NMR), and multiple reaction monitoring (MRM) based LC-MS/MS, using putative steroid glucuronide assignments (Lutz et-al).
REDIchips for Applied Quantitation of Alkaloids
REDIchips provide a rapid and reproducible workflow for the detection and quantitation of small molecules such as ergonovine. Ergonovine is an ergot alkaloid produced by an endophytic fungus that forms a symbiotic relationship with Ipomea tricolor (Morning Glory). This alkaloid has multiple pharmaceutical applications for inducing blood clotting and muscle contractions. In this work, we use REDIchips to detect and quantify ergonovine directly from extracts of whole plant homogenates compared to traditional methods of quantitation.
REDIchips for Rapid Quantitation of Low Molecular Weight Pharmaceutical Drugs Using Laser Desorption Ionization Mass Spectrometry
MALDI-MS has significantly transformed large molecule analysis, but is limited in analysis of low molecular weight compounds, due to the matrix cluster peaks in the low mass regions of MALDI mass spectra. In this work, the performance of nanopost array devices (REDIchips) in quantitating small molecule pharmaceutical drugs in neat solutions and from biofluids is investigated.
Nanostructured Laser Desorption Ionization Device for Small Molecule Analysis
MALDI-MS has significantly transformed large molecule analysis, but is limited in analysis of low molecular weight compounds, due to the matrix cluster peaks in the low mass regions (below 500 Da) of MALDI mass spectra. Successful quantitation of small molecules with MALDI-MS requires careful choice of an appropriate matrix for a given molecule. It also requires using a matrix:sample ratio that minimizes matrix clusters, which complicate the resulting spectra due matrix peaks coinciding with analyte peaks, or suppression of analyte signal. These issues are eliminated by using a matrix-free approach where nanopost arrays are utilized for sample desorption, vaporization and ionization.