I. Select A Kit
ProteaPrep Cell Lysis Kits are available in a variety of formulations. Each kit type utilizes a different surfactant type. A kit should be selected based on your sample type, sensitivity needs, and downstream application.
A 10% ProteaGel with 5% stacking gel with E. Coli lysate load of 15 µL from each ProteaPrep Cell Lysis Kit. Download our full Cell Lysis Kit selection guide here.
II. Digestion / Fractionation / Cleavage
ProteaPrep Cell Lysis Kits are compatible with a variety of bottom-up proteomic sample preparation methods, including solution and gel based techniques. By simply cleaving the labile surfactants and using Protea digestion enzymes and protocols, you can begin digestion without compromising your sample with extensive clean-up measures. Fractionation techniques such as ProteaPrep SCX MudPIT help you make sense of the details of your complex samples and improve your overall mass spec resolution.